Journal: Molecular oral microbiology
Article Title: Filifactor alocis collagenase can modulate apoptosis of normal oral keratinocytes
doi: 10.1111/omi.12163
Figure Lengend Snippet: Bacterial Strains and Plasmids Used In This Study
Article Snippet: F. alocis cultures were grown anaerobically at 37°C, and maintained in an anaerobic chamber (Coy Manufacturing) with 10% H 2 , 10% CO 2 , and 80% N 2 . table ft1 table-wrap mode="anchored" t5 caption a7 Bacterial Strains Description Source Filifactor alocis ATCC 35896 Wild-type strain Aruni et al ., (2011) Escherichia colI Top 10 Genotype: F- mcrA Δ(mrr- hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacΧ74 recA1 araD139 Δ(ara- leu)7697 galU galK rpsL (StrR) endA1 nupG Life technologies E. coli BL21 StarTM (DE3) Genotype: F- ompT hsdSB (rB- mB-) gal dcm rne131 (DE3) Life technologies pFLL215 PrtFAC (HMPREF0389_00504) in Top 10 E.coli This Study Open in a separate window Bacterial Strains and Plasmids Used In This Study Epithelial Cell Culture Conditions Normal Oral Keratinocytes (NOKs) were obtained as a gift from Dr. Penelope Duerksen- Hughes Laboratory (Division of Biochemistry, Loma Linda University School of Medicine) and grown as previously described ( Piboonniyom et al. , 2003 ).
Techniques: